General Information

Product

TLR6 Antibody

Description

Affinity Purified Toll-like receptor 6 Antibody

Verified Applications

ELISA, IHC, IP, WB

Host

Rabbit

Species Cross Reactivity

Cat, Human, Mouse, Rat,Pig

Immunogen

Synthetic peptide taken within amino acid region 195-225 on human TLR6 protein.

Physical Properties

Quantity

100 µg

Volume

200 µl

Concentration

0.78-0.80 µg/µl in antibody stabilization buffer

Form

Affinity Purified

Clonality

Polyclonal

Storage

-20⁰C for long term storage

Application Protocol

DB

1:20,000

ELISA

1:20,000

Western Blot

1:500

Protein

Uniprot #

Q9Y2C9

Overview

Participates in the innate immune response to Gram-positive bacteria and fungi. Specifically recognizes diacylated and, to a lesser extent, triacylated lipopeptides. Acts via MYD88 and TRAF6, leading to NF-kappa-B activation, cytokine secretion and the inflammatory response. Recognizes mycoplasmal macrophage-activating lipopeptide-2kD (MALP-2), soluble tuberculosis factor (STF), phenol-soluble modulin (PSM) and B.burgdorferi outer surface protein A lipoprotein (OspA-L) cooperatively with TLR2.

Molecular Function

Receptor, diacyl lipopeptide binding, transmembrane signaling receptor activity

Subcellular Location

Cell membrane; Single-pass type I membrane protein, Cytoplasmic vesicle > phagosome membrane; Single-pass type I membrane protein

Expression

Detected in monocytes, CD11c+ immature dendritic cells, plasmacytoid pre-dendritic cells and dermal microvessel endothelial cells.

Structure

Binds MYD88 via their respective TIR domains. Heterodimer with TLR2 via their respective extracellular domains (By similarity). Interacts with CD36, following CD36 stimulation by oxLDL or amyloid-beta 42, and forms a heterodimer with TLR4. The trimeric complex is internalized and triggers inflammatory response. LYN kinase activity facilitates TLR4-TLR6 heterodimerization and signal initation.

Alternative Nomenclature

CD286 antibody

TLR 6 antibody

Toll like receptor 6 precursor antibody