General Information | |
Product | RIPK2 Antibody Affinity Purified |
Description | Affinity Purified Receptor-interacting Serine/Threonine-protein kinase 2 Antibody |
Verified Applications | ELISA, IMM, WB |
Host | Rabbit |
Species Cross Reactivity | Bovine, Human, Pig, Monkey |
Immunogen | Synthetic peptide taken within amino acid region 410-460 on human RIPK2 protein. |
Accession # | GenBank: AAQ89173.1 |
Physical Properties | |
Quantity | 100 µg |
Volume | 200 µl |
Concentration | 0.55 µg/µl in antibody stabilization buffer |
Form | Affinity Purified |
Clonality | Polyclonal |
Storage | -20⁰C for long term storage |
Application Protocol |
DB | 1:10,000 |
ELISA | 1:10,000 |
Immunoprecipitation | 1:150 |
Western Blot | 1:500 |
Protein |
Uniprot # | O43353 |
Overview | Serine/threonine/tyrosine kinase that plays an essential role in modulation of innate and adaptive immune responses. Upon stimulation by bacterial peptidoglycans, NOD1 and NOD2 are activated, oligomerize and recruit RIPK2 through CARD-CARD domains. Contributes to the tyrosine phosphorylation of the guanine exchange factor ARHGEF2 through Src tyrosine kinase leading to NF-kappaB activation by NOD2. Once recruited, RIPK2 autophosphorylates and undergoes "Lys-63"-linked polyubiquitination by E3 ubiquitin ligases XIAP, BIRC2 and BIRC3. The polyubiquitinated protein mediates the recruitment of MAP3K7/TAK1 to IKBKG/NEMO and induces "Lys-63"-linked polyubiquitination of IKBKG/NEMO and subsequent activation of IKBKB/IKKB. In turn, NF-kappa-B is released from NF-kappa-B inhibitors and translocates into the nucleus where it activates the transcription of hundreds of genes involved in immune response, growth control, or protection against apoptosis. Plays also a role during engagement of the T-cell receptor (TCR) in promoting BCL10 phosphorylation and subsequent NF-kappa-B activation. |
Molecular Function | ATP binding; CARD domain binding; LIM domain binding; non-membrane spanning protein tyrosine kinase activity; Protein homodimerization activity; protein serine/threonine kinase activity; signal transducer activity |
Subcellular Location | Cytoplasm |
Expression | Detected in heart, brain, placenta, lung, peripheral blood leukocytes, spleen, kidney, testis, prostate, pancreas and lymph node. |
Structure | Found in a signaling complex consisting of at least ARHGEF2, NOD2 and RIPK2. Interacts with ARHGEF2; the interaction mediates tyrosine phosphorylation of RIPK2 by Src kinase CSK. Binds to CFLAR/CLARP and CASP1 via their CARD domains. Binds to BIRC3/c-IAP1 and BIRC2/c-IAP2, TRAF1, TRAF2, TRAF5 and TRAF6. May be a component of both the TNFRSF1A and TNRFSF5/CD40 receptor complex. Interacts with NOD1. Interacts (via CARD domain) with NOD2 (via CARD domain). Interacts with MAP3K4; this interaction sequesters RIPK2 from the NOD2 signaling pathway. Interacts with IKBKG/NEMO. The polyubiquitinated protein interacts with MAP3K7/TAK1. Interacts with XIAP/BIRC4. Interacts with NLRP10. Interacts with CARD9. |
Alternative Nomenclature | CARD carrying kinase antibody CARD CONTAINING ICE ASSOCIATED KINASE antibody CARD containing interleukin 1 beta converting enzyme associated kinase antibody CARDIAK antibody CLARP kinase antibody GIG 30 antibody Growth inhibiting gene 30 antibody Receptor interacting protein 2 antibody Receptor interacting serine threonine kinase 2 RICK antibody RIP-like-interacting CLARP kinase antibody |
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