General Information

Product

C4a Antibody

Verified Applications

ELISA, IHC, IP, WB

Host

Rabbit

Species Cross Reactivity

Human, Monkey, 80% homology with rat

Immunogen

Synthetic peptide taken within amino acid region 1220-1270 on human complement C4-A preprotein.

Physical Properties

Quantity

100 µg

Volume

200 µl

Concentration

0.6 µg/µl in antibody stabilization buffer

Immunoglobulin

IgG

Form

Affinity Purified

Clonality

Polyclonal

Storage

-20⁰C for long term storage

Application Protocol

ELISA

1:10,000

Immunoprecipitation

1:200

Western Blot

1:500

Protein

Uniprot #

P0C0L4

Overview

Non-enzymatic component of C3 and C5 convertases and thus essential for the propagation of the classical complement pathway. Covalently binds to immunoglobulins and immune complexes and enhances the solubilization of immune aggregates and the clearance of IC through CR1 on erythrocytes. C4A isotype is responsible for effective binding to form amide bonds with immune aggregates or protein antigens, while C4B isotype catalyzes the transacylation of the thioester carbonyl group to form ester bonds with carbohydrate antigens. Derived from proteolytic degradation of complement C4, C4a anaphylatoxin is a mediator of local inflammatory process. It induces the contraction of smooth muscle, increases vascular permeability and causes histamine release from mast cells and basophilic leukocytes.

Molecular Function

Complement component C1q binding; Endopeptidase inhibitor activity

Subcellular Location

Secreted

Expression

Complement component C4 is expressed at highest levels in the liver, at moderate levels in the adrenal cortex, adrenal medulla, thyroid gland,and the kidney, and at lowest levels in the heart, ovary, small intestine, thymus, pancreas and spleen. The extra-hepatic sites of expression may be important for the local protection and inflammatory response.

Structure

Contains 1 anaphylatoxin-like domain.

Contains 1 NTR domain.

Alternative Nomenclature

Acidic C4 antibody

Acidic complement C4 antibody

Basic C4 antibody

Basic complement C4 antibody

C3 and PZP like alpha 2 macroglobulin domain containing protein 2 antibody

C4 Anaphylatoxin antibody

C4 complement C4d region antibody

C4 propeptide antibody

C4A antibody

CH antibody

Chido form of C4 antibody

CO4 antibody

Complement C4 A antibody

Complement C4 gamma chain antibody

Complement component 4A antibody

Complement component 4A preproprotein antibody

Complement component 4B (Childo blood group) antibody

CPAMD2 antibody

CPAMD3 antibody

RG antibody

Rodgers Form Of C4 antibody