General Information

Product

Phospho-ErbB 2 Antibody

Description

Affinity Purified Receptor tyrosine-protein kinase erB-2 (HER2) Antibody

Verified Applications

ELISA, IP, WB

Host

Rabbit

Species Cross Reactivity

Mouse, Rat

Predicted:Human

Immunogen

Phoshporylated synthetic peptide taken within amino acid region 1100-1150 on human HER-2 protein

Accession #

GenBank: AAA75493.1

Physical Properties

Quantity

100 µg

Volume

200 µl

Concentration

0.50 µg/µl in antibody stabilization buffer

Form

Affinity Purified

Clonality

Polyclonal

Storage

-20⁰C for long term storage

Application Protocol

DB

1:10,000

ELISA

1:10,000

Immunoprecipitation

1:200

Western Blot

1:500

Protein

Uniprot #

P04626

Overview

Protein tyrosine kinase that is part of several cell surface receptor complexes, but that apparently needs a coreceptor for ligand binding. Essential component of a neuregulin-receptor complex, although neuregulins do not interact with it alone. GP30 is a potential ligand for this receptor. Regulates outgrowth and stabilization of peripheral microtubules (MTs).

Molecular Function

Activator, Kinase, Receptor, Transferase, Tyrosine-protein kinase

Subcellular Location

Isoform 1: Cell membrane; Single-pass type I membrane protein, Cytoplasm>perinuclear region, Nucleus. Isoform 2: Cytoplasm, Nucleus. Isoform 3: Cytoplasm, Nucleus.

Expression

Expressed in a variety of tumor tissues including primary breast tumors and tumors from small bowel, esophagus, kidney and mouth.

Structure

Homodimer. Heterodimer with EGFR, ERBB3 and ERBB4. Part of a complex with EGFR and either PIK3C2A or PIK3C2B. May interact with PIK3C2B when phosphorylated on Tyr-1196. Interacts with PRKCABP and PLXNB1. Interacts (when phosphorylated on Tyr-1248) with MEMO1. Interacts with MUC1; the interaction is enhanced by heregulin (HRG).

Alternative Nomenclature

Verb b2 erythroblastic leukemia viral oncogene homolog 2, neuro/glioblastoma derived oncogene homolog antibody

c erb B2/neu protein antibody

CD340 antibody

CerbB2 antibody

Erb B2 antibody

Erythroblastosis Virus antibody

HER 2/neu antibody

Herstatin antibody

Metastatic lymph node gene 19 protein antibody

MLN19 antibody

NEU antibody

Neuro Glioblastoma Derived Oncogene Homolog antibody

NGL antibody

p185 ErbB2 antibody

Receptor Protein Tyrosine Kinase ErbB2 Precursor antibody

TKR1 antibody

Tyrosine kinase-type cell surface receptor HER2 antibody

v erb b2 erythroblastic leukemia viral oncogene homolog 2 neuro/glioblastoma derived oncogene homolog (avian) antibody

verbb2 antibody