General Information

Product

ENPP2 Antibody

Description

Affinity Purified ectonucleotide pyrophosphatase/phosphodiesterase 2 (Autotaxin) Antibody C-epitope

Verified Applications

ELISA, IHC, IP, WB

Host

Rabbit

Species Cross Reactivity

Human, Monkey, Mouse, Rat

Immunogen

Synthetic peptide taken within amino acid region 778-818 on human Ectonucleotide pyrophosphatase/phosphodiesterase 2 (autotaxin) protein.

Accession #

GenBank: EAW91988.1

Physical Properties

Quantity

100 µg

Volume

200 µl

Concentration

0.75-0.95 µg/µl in antibody stabilization buffer

Form

Affinity Purified

Clonality

Polyclonal

Determinant

C-epitope

Storage

-20⁰C for long term storage

Application Protocol

Dot Blot

1:10,000

ELISA

1:10,000

Immunoprecipitation

1:200

Western Blot

1:250

Protein

Uniprot #

UPI0000EE3297

Overview

Hydrolyzes lysophospholipids to produce lysophosphatidic acid (LPA) in extracellular fluids. Major substrate is lysophosphatidylcholine. Also can act on sphingosylphosphphorylcholine producing sphingosine-1-phosphate, a modulator of cell motility. Can hydrolyze, in vitro, bis-pNPP, to some extent pNP-TMP, and barely ATP. Involved in several motility-related processes such as angiogenesis and neurite outgrowth. Acts as an angiogenic factor by stimulating migration of smooth muscle cells and microtubule formation. Stimulates migration of melanoma cells, probably via a pertussis toxin-sensitive G protein. May have a role in induction of parturition. Possible involvement in cell proliferation and adipose tissue development. Tumor cell motility-stimulating factor.

Molecular Function

alkylglycerophosphoethanolamine phosphodiesterase activity; calcium ion binding; hydrolase activity; lysophospholipase activity; nucleic acid binding; nucleotide diphosphatase activity; phosphodiesterase I activity; polysaccharide binding; scavenger receptor activity; transcription factor binding; zinc ion binding

Subcellular Location

Secreted

Expression

Predominantly expressed in brain, placenta, ovary, and small intestine. Expressed in a number of carcinomas such as hepatocellular and prostate carcinoma, neuroblastoma and non-small-cell lung cancer. Expressed in body fluids such as plasma, cerebral spinal fluid (CSF), saliva, follicular and amniotic fluids. Not detected in leukocytes. Isoform 1 is more highly expressed in peripheral tissues than in the central nervous system (CNS). Adipocytes only express isoform 1. Isoform 3 is more highly expressed in the brain than in peripheral tissues.

Structure

Belongs to the nucleotide pyrophosphatase/phosphodiesterase family.

Contains 2 SMB (somatomedin-B) domains.

Alternative Nomenclature

ATX antibody

ATX X antibody

Autotaxin antibody

Autotaxin t antibody

Ectonucleotide pyrophosphatase/phosphodiesterase 2 antibody

Enpp2 antibody

Extracellular lysophospholipase D antibody

FLJ26803 antibody

LysoPLD antibody

NPP2 antibody

PD IALPHA antibody

PDNP2 antibody

Phosphodiesterase I alpha antibody

Plasma lysophospholipase D antibody