General Information |
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Product |
Phospho-FGFR1 Antibody |
Description | Affinity Purified Phospho-Fibroblast Growth Factor Receptor 1 Antibody |
Verified Applications | ELISA, IP, WB |
Host | Rabbit |
Species Cross Reactivity | Human, Rat |
Immunogen | Synthetic peptide taken within amino acid region 640-690 on human basic fibroblast growth factor receptor protein 1. |
Accession # | GenBank: AAA75007.1 |
Physical Properties | |
Quantity |
100 µg |
Volume | 200 µl |
Concentration | 0.55-0.75 µg/µl in antibody stabilization buffer |
Immunoglobulin | IgG |
Form | Affinity Purified |
Clonality | Polyclonal |
Storage | -20⁰C for long term storage |
Application Protocol |
ELISA |
1:10,000 |
Immunoprecipitation | 1:150 |
Western Blot | 1:500 |
Protein |
Uniprot # |
P11362 |
Overview | Tyrosine-protein kinase that acts as cell-surface receptor for fibroblast growth factors and plays an essential role in the regulation of embryonic development, cell proliferation, differentiation and migration. Required for normal mesoderm patterning and correct axial organization during embryonic development, normal skeletogenesis and normal development of the gonadotropin-releasing hormone (GnRH) neuronal system. Phosphorylates PLCG1, FRS2, GAB1 and SHB. Ligand binding leads to the activation of several signaling cascades. Activation of PLCG1 leads to the production of the cellular signaling molecules diacylglycerol and inositol 1,4,5-trisphosphate. Phosphorylation of FRS2 triggers recruitment of GRB2, GAB1, PIK3R1 and SOS1, and mediates activation of RAS, MAPK1/ERK2, MAPK3/ERK1 and the MAP kinase signaling pathway, as well as of the AKT1 signaling pathway. Promotes phosphorylation of SHC1, STAT1 and PTPN11/SHP2. In the nucleus, enhances RPS6KA1 and CREB1 activity and contributes to the regulation of transcription. FGFR1 signaling is down-regulated by IL17RD/SEF, and by FGFR1 ubiquitination, internalization and degradation. |
Molecular Function | ATP-binding; Fibroblast growth factor binding; Heparin binding; Protein Tyrosine Kinase activity |
Subcellular Location | Cell membrane; Single-pass type I membrane protein. Nucleus. Cytoplasm › cytosol. Cytoplasmic vesicle |
Expression | Detected in astrocytoma, neuroblastoma and adrenal cortex cell lines. Some isoforms are detected in foreskin fibroblast cell lines, however isoform 17, isoform 18 and isoform 19 are not detected in these cells. |
Structure | Monomer. Homodimer after ligand binding. Interacts predominantly with FGF1 and FGF2, but can also interact with FGF3, FGF4, FGF5, FGF6, FGF8, FGF10, FGF19, FGF21, FGF22 and FGF23 (in vitro). Ligand specificity is determined by tissue-specific expression of isoforms, and differences in the third Ig-like domain are crucial for ligand specificity. Affinity for fibroblast growth factors (FGFs) is increased by heparan sulfate glycosaminoglycans that function as coreceptors. Likewise, KLB increases the affinity for FGF19, FGF21 and FGF23. Interacts (phosphorylated on Tyr-766) with PLCG1 (via SH2 domains). Interacts with FRS2. Interacts (via C-terminus) with NEDD4 (via WW3 domain). Interacts with KL (By similarity). Interacts with SHB (via SH2 domain) and GRB10. Interacts with KAL1; this interaction does not interfere with FGF2-binding to FGFR1, but prevents binding of heparin-bound FGF2. Interacts with SOX2 and SOX3. |
Alternative Nomenclature | Basic fibroblast growth factor receptor 1 antibody bFGF R antibody C FGR antibody CD 331 antibody CEK antibody FGFBR antibody FGFR 1 antibody Fibroblast growth factor receptor 1 antibody FLG antibody FLG protein antibody FLT 2 antibody FMS like tyrosine kinase 2 antibody Fms related tyrosine kinase 2 Pfeiffer syndrome antibody Fms-like tyrosine kinase 2 antibody HBGFR antibody Heparin binding growth factor receptor antibody HH2 antibody HRTFDS antibody Hydroxyaryl protein kinase antibody KAL 2 antibody MFR antibody N sam tyrosine kinase antibody N-SAM antibody OGD antibody Proto-oncogene c-Fgr antibody Tyrosylprotein kinase antibody |
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