General Information

Product

Bcl6 Antibody

Description

Affinity Purified B-Cell lymphoma 6 proteinAntibody C-epitope

Verified Applications

ELISA, IHC, IP, WB

Host

Rabbit

Species Cross Reactivity

Human, Monkey, Mouse, Rat

Immunogen

Synthetic peptide taken within amino acid region 610-660on human B-cell lymphoma 6 protein isoform 1.

Accession #

NCBI: NP_001124317

Physical Properties

Quantity

100 µg

Volume

200 µl

Concentration

0.6 µg/µl in antibody stabilization buffer

Form

Affinity Purified

Clonality

Polyclonal

Determinant

C-epitope

Storage

-20⁰C for long term storage

Application Protocol

DB

1:10,000

ELISA

1:10,000

Immunoprecipitation

1:150

Western Blot

1:500

Protein

Uniprot #

P41182

Overview

Transcriptional repressor mainly required for germinal center (GC) formation and antibody affinity maturation which has different mechanisms of action specific to the lineage and biological functions. Forms complexes with different corepressors and histone deacetylases to repress the transcriptional expression of different subsets of target genes. Represses its target genes by binding directly to the DNA sequence 5"-TTCCTAGAA-3" (BCL6-binding site) or indirectly by repressing the transcriptional activity of transcription factors. In GC B-cells, represses genes that function in differentiation, inflammation, apoptosis and cell cycle control, also autoregulates its transcriptional expression and up-regulates, indirectly, the expression of some genes important for GC reactions, such as AICDA, through the repression of microRNAs expression, like miR155. An important function is to allow GC B-cells to proliferate very rapidly in response to T-cell dependent antigens and tolerate the physiological DNA breaks required for immunglobulin class switch recombination and somatic hypermutation without inducing a p53/TP53-dependent apoptotic response. In follicular helper CD4+ T-cells (T(FH) cells), promotes the expression of T(FH)-related genes but inhibits the differentiation of T(H)1, T(H)2 and T(H)17 cells. Also required for the establishment and maintenance of immunological memory for both T- and B-cells. Suppresses macrophage proliferation through competition with STAT5 for STAT-binding motifs binding on certain target genes, such as CCL2 and CCND2. In response to genotoxic stress, controls cell cycle arrest in GC B-cells in both p53/TP53-dependedent and -independent manners. Besides, also controls neurogenesis through the alteration of the composition of NOTCH-dependent transcriptional complexes at selective NOTCH targets, such as HES5, including the recruitment of the deacetylase SIRT1 and resulting in an epigenetic silencing leading to neuronal differentiation.

Molecular Function

Chromatin Binding; Actin Cytoskeleton organization; Cellular response to DNA damage stimulus; Negative regulation of cell growth; Activator; Repressor

Subcellular Location

Nucleus

Expression

Expressed in germinal center T- and B-cells and in primary immature dendritic cells.

Structure

Homodimer.

Alternative Nomenclature

B cell CLL/lymphoma 6 antibody

B cell lymphoma 6 protein antibody

B-cell lymphoma 5 protein antibody

BCL5 antibody

BCL6A antibody

cys his2 zinc finger transcription factor antibody

LAZ3 antibody

Lymphoma associated zinc finger gene on chromosome 3 antibody

Protein LAZ-3 antibody

ZBTB27 antibody

Zinc finger and BTB domain-containing protein 27 antibody

Zinc finger protein 51 antibody

zinc finger transcription factor BCL6S antibody

ZNF 51 antibody