FabGennix/磷酸化MERTK抗体/PMKT-140AP-免疫在线蚂蚁淘旗下平台

商品详细FabGennix/磷酸化MERTK抗体/PMKT-140AP

FabGennix/磷酸化MERTK抗体/PMKT-140AP

商品编号： PMKT-140AP

品牌： FabGennix Inc

市场价： ¥7000.00

美元价： 3150.00

产地：美国(厂家直采)

公司：

产品分类：功能性抗体

公司分类： Functional_antibody

联系Q Q： 3392242852

电话号码： 4000-520-616

电子邮箱： info@ebiomall.com

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商品介绍

General Information
Product	Phospho-MERTK Antibody
Description	Affinity Purified Phosphorylated Tyrosine-protein kinase Mer Antibody
Verified Applications	CM, ELISA, FC, ICC, IF, IHC, WB
Host	Rabbit
Species Cross Reactivity	Human, Monkey, Mouse, Rat
Immunogen	Synthetic peptide corresponding to amino acids 746-757 ofHuman MERTK. Sequence: CKKIY_pSGDY_pY_pRQG
Accession #	NCBI: NP_075232.1
Physical Properties
Quantity	100 µg
Volume	200 µl
Concentration	0.63 µg/µl in antibody stabilization buffer
Form	Affinity Purified
Clonality	Polyclonal
Storage	-20⁰C for long term storage
Application Protocol
Confocal Microscopy	1:250
DB	1:10,000
ELISA	1:10,000
Immunocytochemistry	1:250
Immunofluorescence	1:250
Immunohistochemistry	1:250
Western Blot	1:750. Observed band of 180kDa
Protein
Uniprot #	P57097
Overview	Receptor tyrosine kinase that transduces signals from the extracellular matrix into the cytoplasm by binding to several ligands including LGALS3, TUB, TULP1 or GAS6. Regulates many physiological processes including cell survival, migration, differentiation and phagocytosis of apoptotic cells (efferocytosis). Ligand binding at the cell surface induces autophosphorylation of MERTK on its intracellular domain that provides docking sites for downstream signaling molecules.
Molecular Function	Kinase, Receptor, Transferase, Tyrosine-protein kinase
Subcellular Location	Membrane; Single-pass type I membrane protein
Structure	Interacts (upon activation) with TNK2; stimulates TNK2 autophosphorylation. Interacts (via N-terminus) with extracellular ligands LGALS3, TUB, TULP1 and GAS6. Interacts with VAV1 in a phosphotyrosine-independent manner.
Alternative Nomenclature	c MER antibody c mer proto oncogene tyrosine kinase Eyk MER MERPEN Mertk MERTK c-mer proto-oncogene tyrosine kinase nmf12 Nyk Proto oncogene tyrosine protein kinase MER Receptor tyrosine kinase MerTK RP38 STK kinase Tyrosine-protein kinase Mer

Protein information supplied by UniProt

FabGennix Phospho-MerTK Antibody has been used in 26Publications

Applications: Confocal microscopy, Flow cytometry, Immunocytochemistry, Immunohistochemistry Immunofluorescence, and Western blot

Reactivity: Human, Mouse, and Rat

ZCai, Bishuang, et al. "MerTK signaling in macrophages promotes the synthesis of inflammation resolution mediators by suppressing CaMKII activity." Sci. Signal. 11.549 (2018).

PMID:30254055

Applications:Western Blot

Reactivity: Human

Zhang, Bing, et al. "Mer receptor tyrosine kinase negatively regulates lipoteichoic acid-induced inflammatory response via PI3K/Akt and SOCS3." Molecular immunology 76 (2016).

PMID: 27419619

Applications: Western Blot

Reactivity: Mouse

Das, Amitava, et al. "Correction of MFG-E8 resolves inflammation and promotes cutaneous wound healing in diabetes." The Journal of Immunology 196.12 (2016).

PMID: 27194784

Applications: Western Blot

Reactivity: Mouse

Cai, Bishuang, et al. "MerTK cleavage limits proresolving mediator biosynthesis and exacerbates tissue inflammation." Proceedings of the National Academy of Sciences 113.23 (2016).

PMID: 27199481

Applications: Western Blot

Reactivity: Mouse

Ginisty, Aurélie, et al. "Evidence for a Subventricular Zone Neural Stem Cell Phagocytic Activity Stimulated by the Vitamin K‐Dependent Factor Protein S." STEM CELLS 33.2 (2015).

PMID: 25308179

Applications: Western Blot

Reactivity: Rat

Nguyen, Khanh-Quynh N., et al. "Overexpression of MERTK receptor tyrosine kinase in epithelial cancer cells drives efferocytosis in a gain-of-function capacity." Journal of Biological Chemistry 289.37 (2014).

PMID: 25074939

Applications: Western Blot

Reactivity: Human

Law, Ah-Lai, et al. "Cleavage of Mer Tyrosine Kinase (MerTK) from the Cell Surface Contributes to the Regulation of Retinal Phagocytosis." Journal of Biological Chemistry (2014).

PMID: 25538233

Applications: Western Blot

Reactivity: Mouse

Suleiman, Lutfi, Claude Négrier, and Habib Boukerche. "Protein S: A multifunctional anticoagulant vitamin K-dependent protein at the crossroads of coagulation, inflammation, angiogenesis, and cancer."Critical Reviews in oncology/hematology88.3 (2013).

PMID: 23065156

Applications: Western Blot

Reactivity: Mouse

Wang, Y, et al. "Mer Receptor Tyrosine Kinase Promotes Invasion and Survival In Glioblastoma Multiforme."Oncogene32.7 (2013): 872-882.

PMID: 22469987

Applications: Confocal Microscopy, Immunofluorescence, Immunohistochemistry, Western Blot

Reactivity: Human

Choi, Ji-Yeon, et al. "Upregulation of Mer receptor tyrosine kinase signaling attenuated lipopolysaccharide-induced lung inflammation." Journal of Pharmacology and Experimental Therapeutics 344.2 (2013).

PMID: 23197771

Applications: Western Blot

Reactivity: Mouse

Yefimova, Marina G. et al. “A Chimerical Phagocytosis Model Reveals the Recruitment by Sertoli Cells of Autophagy for the Degradation of Ingested Illegitimate Substrates.” Autophagy9.5 (2013).

PMCID: 3669177

Applications: Confocal Microscopy, Immunocytochemistry, Immunofluorescence, Western Blot

Reactivity: Human

Click Here for the complete list of all FabGennix Phospho-MerTK Antibody publications

Publishing research using PMKT-140AP? Please let us know, we can cite the reference in this datasheet.

To view the Specification Sheet for Phospho-MerTKAntibody, please click the link below:

Phospho-MerTK Antibody| FabGennix

I run cell lysates from different human cell lines corresponding to 50 ug total protein (lysis buffer 25 mM Tris pH 7.4, 150 mM NaCl, 5 mM EDTA, 10% glycerol, 1% Triton X-100 with protease and phosphatase inhibitors) under reducing conditions on a 8% gel followed by blotting to a PVDF membrane. As blocking reagent I used 5% skim Milk in 50 mM Tris pH 8, 150 mM NaCl, 0.5% Tween-20, 1 h RT. Followed by 3 washes with TBST buffer (50 mM Tris pH 8, 150 mM NaCl, 0.1% Tween-20).

I incubated the blot with the above mentioned antibody for overnight at 4 C on rotating shaker diluted 1:750 in BSA buffer (5% BSA in 50 mM Tris pH 8, 150 mM NaCl, 0.5% Tween-20 ).

Next day, followed by 6 washes with 50 mM Tris pH 8, 150 mM NaCl, 0.5% Tween-20. As a secondary antibody I used a anti-rabbit HRP conjugate from cell signaling, diluted 1:10000 in blocking buffer. Incubation time again 1 h RT. The membrane was washed 6 times as above and developed using ECL.

After phosphoMer detection, the blot was washed 6 times with 50 mM Tris pH 8, 150 mM NaCl, 0.5% Tween-20 was analysed for total MERTK using the AF891 from R & D system. Similar process was used for total MERTK also.

Results-

When detecting phosphoMer I see several bands from 130 KD to 250 KD. whereas in total MERTK I see a single band.

We know now for sure that:

phospho-MER protein showed some abnormality in the migration on SDS-PAGE, so therefore the proteins will not be exactly at the same place in phospho-lane.

The non-phospho-MER has differences in the MW due to differential glycosylation on the core protein. This was very elegantly published by Doug Graham (who cloned this gene) using some of our reagents

I am not sure about the characteristics of non-phospho-MER antibodiesused in these experiments. Do they recognize all forms of differentially modified MER or just recognize the core proteins and does not recognize the fully mature MER proteins in the samples?

Our non-Phospho-MER antibodies (MKT-101AP, MKT-112AP and MKT-121AP) recognize not only the core proteins but also the glycosylated from different tissues and cells extracts.

In some samples our Phospho-mer antibody (PMKT-140AP) also recognize a smaller (just above 90kDa) band. The MW of these proteins are apparent in relation to the MW standards and cannot be considered as actual size of the proteins. These numbers will vary quite significantly on different pore size gels, and sometimes even when different companies MW markers are used. We also know that calculated MW will also vary when colored and non-color MW makers are compared on the same gel.

I run cell lysates from different human cell lines corresponding to 50 ug total protein (lysis buffer 25 mM Tris pH 7.4, 150 mM NaCl, 5 mM EDTA, 10% glycerol, 1% Triton X-100 with protease and phosphatase inhibitors) under reducing conditions on a 8% gel followed by blotting to a PVDF membrane. As blocking reagent I used 5% skim Milk in 50 mM Tris pH 8, 150 mM NaCl, 0.5% Tween-20, 1 h RT. Followed by 3 washes with TBST buffer (50 mM Tris pH 8, 150 mM NaCl, 0.1% Tween-20).

I incubated the blot with the above mentioned antibody for overnight at 4 C on rotating shaker diluted 1:750 in BSA buffer (5% BSA in 50 mM Tris pH 8, 150 mM NaCl, 0.5% Tween-20 ).

Next day, followed by 6 washes with 50 mM Tris pH 8, 150 mM NaCl, 0.5% Tween-20. As a secondary antibody I used a anti-rabbit HRP conjugate from cell signaling, diluted 1:10000 in blocking buffer. Incubation time again 1 h RT. The membrane was washed 6 times as above and developed using ECL.

After phosphoMer detection, the blot was washed 6 times with 50 mM Tris pH 8, 150 mM NaCl, 0.5% Tween-20 was analysed for total MERTK using the AF891 from R & D system. Similar process was used for total MERTK also.

Results-

When detecting phosphoMer I see several bands from 130 KD to 250 KD. whereas in total MERTK I see a single band.

We know now for sure that:

phospho-MER protein showed some abnormality in the migration on SDS-PAGE, so therefore the proteins will not be exactly at the same place in phospho-lane.

The non-phospho-MER has differences in the MW due to differential glycosylation on the core protein. This was very elegantly published by Doug Graham (who cloned this gene) using some of our reagents

I am not sure about the characteristics of non-phospho-MER antibodiesused in these experiments. Do they recognize all forms of differentially modified MER or just recognize the core proteins and does not recognize the fully mature MER proteins in the samples?

Our non-Phospho-MER antibodies (MKT-101AP, MKT-112AP and MKT-121AP) recognize not only the core proteins but also the glycosylated from different tissues and cells extracts.

In some samples our Phospho-mer antibody (PMKT-140AP) also recognize a smaller (just above 90kDa) band. The MW of these proteins are apparent in relation to the MW standards and cannot be considered as actual size of the proteins. These numbers will vary quite significantly on different pore size gels, and sometimes even when different companies MW markers are used. We also know that calculated MW will also vary when colored and non-color MW makers are compared on the same gel.

Accessories

Product

Note

Status

Price

Phospho-MERTK Antibody FITC

PMKT-FITC

$395.00*

Phospho-MERTK Antibody BIOTIN

PMKT-BIOTIN

$395.00*

Phospho-MERTK Blocking Peptide

P-PMKT

$180.00*

Phospho-MERTK Positive Control

PC-PMKT

$205.00*

*

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Display accessory details

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品牌介绍

苏州蚂蚁淘Fabgennix授权代理业务。Fabgennix授权代理， Fabgennix北京代理， Fabgennix华东代理，Fabgennix华北代理，Fabgennix华中代理，Fabgennix华南代理。 Fabgennix 我们提供高质量的研究工具，专家技术支持，相关的准确数据以及快速的全球运输。我们不断在我们的库存中添加新产品，包括一级和二级抗体，试剂，试剂盒，多肽和阳性对照。我们所有的抗体都在内部开发和表征。我们为我们的Single Source Antibodies™感到自豪。部分产品的详细列表：Fabgennix授权代理货号产品价格 FGI-1989 StripOBuffer Western Blot Stripping Buffer $150.00 HRP-1060 Rabbit Anti-Chicken IgG HRP $150.00 FGI-1943 SolObuffer Cell Lysis Kit $175.00 FGI-1963 DiluOBuffer - Antibody Dilution Buffer $185.00 HIL2-E96.1 Human IL-2 ELISA Kit $495.00